Biotechnology: Principles and Processes MCQ Questions:
Below is the list of all the Important Questions of Biotechnology: Principles and Processes MCQ for NEET. Here you can get the MCQs with answers and also download PDFs provided for each topic for extra MCQ on Biotechnology: Principles and Processes to practice more.
Biotechnology: Principles and Processes
1. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
(1) The larger the fragment size, the farther it moves
(2) The smaller the fragment size, the farther it moves
(3) Positively charged fragments move to the farther end
(4) Negatively charged fragments do not move
Answer: 2
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2. The correct order of steps in the Polymerase Chain Reaction (PCR) is_____
(1) Extension, Denaturation, Annealing
(2) Annealing, Extension, Denaturation
(3) Denaturation, Extension, Annealing
(4) Denaturation, Annealing, Extension
Answer: 4
3. A transgenic crop of the future, golden rice has which of the following improved traits?
(1) High lysine (essential amino acid) content
(2) Insect resistance
(3) High protein content
(4) High vitamin-A content
Answer: 4
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4. Bt toxin kills the insect by________
(1) Blocking the nerve conduction
(2) Damaging the surface of the trachea
(3) By creating pores in the tracheal system
(4) By creating a pore in the midgut.
Answer: 4
5. Transgenic rice (Golden rice) has been developed for increased content of________
(1) Vitamin A
(2) Vitamin B1
(3) Vitamin C
(4) Vitamin D
Answer: 1
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6. Which of the following is produced commercially using genetically engineered bacteria?
(1) thyroxine
(2) human insulin
(3) testosterone
(4) penicillin
Answer: 2
7. What is the process by which crop plants are enriched with certain desirable nutrients?
(1) crop protection
(2) breeding
(3) bio-fortification
(4) bio-remediation
Answer: 3
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8. Which of the following is true for Bt-cotton?
(1) It is used to kill the virus
(2) It kills both targeted and non-targeted insects
(3) It kills only targeted insect
(4) It kills the only fungal pathogen
Answer: 3
9. A gene whose expression helps to identify transformed cell is known as___________
(1) Selectable marker
(2) Vector
(3) Plasmid
(4) Structural gene
Answer: 1
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10.Which of the following is not a direct method of gene transfer in plants:-
(1) Agrobacterium tumefaciens
(2) Gene gun method
(3) Biolistic method
(4) Electroporation
Answer: 1
11. Which one is used as a vector for gene transfer cloning gene?
(1) Salmonella typhimurium DNA
(2) Ti plasmid
(3) Antibiotic resistance
(4) Ori minus pBR 322
Answer: 2
12. Which one of the following techniques are used to produce the GM crops?
(1) Micropropagation
(2) Somatic hybridization
(3) r-DNA technology
(4) Crossbreeding
Answer: 3
13. RNAi results in________
(1) Silencing of mRNA translation
(2) Silencing of a specific mRNA due to
complementary ds RNA molecule.
(3) Silencing of mRNA molecule
(4) Silencing of DNA for mRNA transcription
Answer: 2
14. The process of separation and purification of expressed protein before marketing is called______
(1) Downstream processing
(2) Bioprocessing
(3) Postproduction processing
(4) Upstream processing
Answer: 1
15. A gene whose expression helps to identify the transformed cell is known as______
(1) Vector
(2) Plasmid
(3) Structural gene
(4) Selectable marker
Answer: 4
16. Which one of the given options correctly identifies its certain component(s)?
(1) Ori - original restriction enzyme
(2) Rop - Reduced osmotic pressure
(3) Hind I, EcoR I - selectable markers
(4) AmpR, tetR - antibiotic resistance genes
Answer: 4
17. There is a restriction endonuclease called EcoRI. What does 'co part in it stand for?
(1) Coelom
(2) Coli
(3) Colon
(4) Coenzyme
Answer: 2
18. Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands?
5'____________GAATTC____________3'
5'____________CTTAAG____________3'
What is so special shown in it?
(1) Replication completed
(2) Deletion mutation
(3) Start codon at the 5' end
(4) Palindromic sequence of base pairs
Answer: 4
19. A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using_______
(1) Polymerase-III
(2) Ligase
(3) Eco RI
(4) Taq polymerase
Answer: 2
20. Which of the following is not a component of downstream processing?
(1) Preservation
(2) Expression
(3) Separation
(4) Purification
Answer: 2
21. GEAC stands for________
(1) Genetic engineering approval committee
(2) Genetic engineering agreement council
(3) Genetic engineering analyzing crop
(4) Genetic engineering agricultural committee
Answer: 1
22. The DNA fragments separated on an agarose gel can be visualized after staining with__________
(1) Bromophenol blue
(b) Acetocarmine
(c) Aniline blue
(d) Ethidium bromide
Answer: 4
23. Which of the following restriction enzymes produces blunt ends?
(a) Eco RV
(2) Sal I
(3) Hind III
(4) Xho I
Answer: 1
24. Stirred-tank bioreactors have been designed for________
(1) availability of oxygen throughout the process
(2) ensuring anaerobic conditions in the culture vessel
(3) purification of the product
(4) addition of preservatives to the product
Answer: 1
25. A foreign DNA and plasmid cut by me restriction endonuclease can be joined to form a recombinant plasmid using_____________
(1) Eco RI
(2) Taq polymerase
(3) Ligase
(4) Polymerase II
Answer: 3
26. Which of the following is not a feature of the plasmid?
(1) Single-stranded
(2) Independent replication
(3) Circular structure
(4) Small, circular double-stranded
Answer: 1
27. Commonly used vectors for human genome sequencing are_____________
(1) T-DNA
(2) BAC vectors
(3) T/C cloning vector
(4) Expression vector
Answer: 2
28. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by_________
(1) Centrifugation
(2) Polymerase Chain Reaction
(3) Electrophoresis
(4) Restriction mapping
Answer: 3
29. An example of gene therapy is______
(1) Production of injectable Hepatitis–B vaccine
(2) Production of vaccines in food crops like potatoes which can be eaten
(3) Introduction of the gene for adenosine deaminase in persons suffering from severe combined immunodeficiency (SCID)
(4) Production of test-tube babies by artificial insemination and implantation of fertilized eggs
Answer: 3
30. Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)________
(1) an antifeedant
(2) a toxic protein
(3) both sense and anti-sense RNA
(4) a particular hormone
Answer: 3