Biotechnology and Its Applications NEET Questions with Solutions :
Biotechnology and Its Applications NEET Questions is a Scoring topic where candidates can attempt to solve a maximum number of accurate questions and increase their overall score count in the NEET exam. Not only in NEET exams but in other competitive exams as well, Biotechnology and Its Applications is considered to be the most Scoring and Simple Chapter. The aim of this Chapter Biotechnology and Its Applications MCQ is to test candidates’ awareness and knowledge regarding Biotechnology and Its Applications.
These NEET Questions on Biotechnology and Its Applications for class 12 Pdf Download will help students score in the upcoming NEET exam and perform better in competitive exams, such as CET, NET, SET. Hence, it is suggested to students to solve all these Biotechnology and Its Applications NEET Questions Pdf Download with their problem-solving skills and cross-check the answers. Also, try to maintain a time limit for each question. Below is the list of all the Important Questions of Biotechnology and Its Applications for NEET. Here you can get the MCQs with answers and also download PDFs provided for each topic for extra MCQ on Biotechnology and Its Applications to practice more.
NEET Questions on Biotechnology and Its Applications for Class 12 :
1. The process of separation and purification of expressed protein before marketing is called :
(1) Downstream processing
(2) Bioprocessing
(3) Postproduction processing
(4) Upstream processing
Answer: 1
2. A gene whose expression helps to identify transformed cells is known as :
(1) Vector
(2) Plasmid
(3) Structural gene
(4) Selectable marker
Answer: 4
3. Which of the following is true for Bt-cotton?
(1) It is used to kill the virus
(2) It kills both targeted and nontargeted insects
(3) It kills only targeted insect
(4) It kills the only fungal pathogen
Answer: 3
4. First plasmid of which bacterium is used as a vector in rDNA technique:-
(1) Salmonella
(2) E.coli
(3) Agrobacterium
(4) Bacillus
Answer: 1
5. The process by which restriction endonuclease cut the DNA molecule:-
(1) Reduction
(2) Oxidation
(3) Hydrolysis
(4) Modification
Answer: 3
6. Restriction endonuclease cut the DNA molecule at which site?
(1) Sugar-phosphate backbone
(2) Between two base pairs
(3) Between sugar and base
(4) Between phosphate and base
Answer: 1
7. In r-DNA technology which part of Ti-plasmid of Agrobacterium is transferred to the host plant?
(1) T-DNA
(2) T-DNA + Vir-region
(3) T-DNA + Ori-region
(4) Vir-region
Answer: 1
8. Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
(1) Retrovirus
(2) Ti plasmid
(3) Lambda phage
(4) PBR 322
Answer: 1
9. In India, the organization responsible for assessing the safety of introducing genetically modified organisms for public use is________
(1) Indian Council of Medical Research (ICMR)
(2) Council for Scientific and Industrial Research (CSIR)
(3) Research Committee on Genetic Manipulation (RCGM)
(4) Genetic Engineering Appraisal Committee (GEAC)
Answer: 4
10. A 'new variety of rice was patented by a foreign company through such varieties have been present in India for a long time. This is related to______
(1) Co-667
(2) Sharbati Sonora
(3) Lerma Rojo
(4) Basmati
Answer: 4
11. Use of bioresources by multinational companies and organizations without authorization from the concerned country and its people is called
(1) Bio-infringement
(2) Biopiracy
(3) Biodegradation
(4) Bioexploitation
Answer: 2
12. The correct order of steps in Polymerase Chain Reaction (PCR) is
(1) Extension, Denaturation, Annealing
(2) Annealing, Extension, Denaturation
(3) Denaturation, Extension, Annealing
(4) Denaturation, Annealing, Extension
Answer: 4
13. The two polypeptides of human insulin are linked together by:-
(1) Hydrogen bonds
(2) Phosphodiester bond
(3) Covalent bond
(4) Disulphide bridges
Answer: 4
14. Which of the following is not a feature of the plasmids?
(1) Independent replication
(2) Circular structure
(3) Transferable
(4) Single-stranded
Answer: 4
15. The Taq polymerase enzyme is obtained from :
(1) Thermus aquaticus
(2) Thiobacillus ferroxidans
(3) Bacillus subtilis
(4) Pseudomonas putida
Answer: 1
16. Which of the following is a restriction endonuclease?
(1) Hind II
(2) Protease
(3) DNase I
(4) RNase
Answer: 1
17. stirred-tank bioreactors have been designed for :
(1) availability of oxygen throughout the process
(2) ensuring anaerobic conditions in the culture vessel
(3) purification of the product
(4) addition of preservatives to the product
Answer: 1
18. GEAC stands for:-
(1) Genetic engineering approval committee
(2) Genetic engineering agreement council
(3) Genetic engineering analyzing crop
(4) Genetic engineering agricultural committee
Answer: 1
19. Which one of the following is a method of gene silencing:-
(1) tRNA
(2) rRNA
(3) RNAi
(4) mRNA
Answer: 3
20. Which of the following is not a direct method of gene transfer in plants:-
(1) Agrobacterium tumefaciens
(2) Gene gun method
(3) Biolistic method
(4) Electroporation
Answer: 1
21. Production of a human protein in bacteria by genetic engineering is possible because_______
(1) Bacterial cells can carry out the RNA splicing reactions
(2) The mechanism of gene regulation is identical in humans and bacteria
(3) The human chromosome can replicate in bacterial cell
(4) The genetic code is universal
Answer: 4
22. Two microbes found to be very useful in genetic engineering are –
(1) Escherichia coli and Agrobacterium tumefaciens
(2) Vibrio cholera and a tailed bacteriophage
(3) Diplococcus sp. and Pseudomonas sp.
(4) Crown gall bacterium and Caenorhabditis elegans
Answer: 1
23. Restriction endonuclease –
(1) Cuts the DNA molecule randomly
(2) Cuts the DNA molecule at specific sites
(3) Restricts the synthesis of DNA inside the nucleus
(4) Synthesizes DNA
Answer: 2
24. Golden rice is a promising transgenic crop. When released for cultivation, it will help in_______
(1) Alleviation of vitamin A deficiency
(2) Pest resistance
(3) Herbicide tolerance
(4) Producing a petrol-like fuel from rice
Answer: 1
25. A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of:-
(1) Pseudomonas
(2) Trichoderma
(3) Xanthomonas
(4) Bacillus
Answer: 1
26. Cry1 endotoxins obtained from Bacillus thuringiensis are effective against:-
(1) Flies
(2) Nematodes
(3) Boll worms
(4) Mosquitoes
Answer: 3
27. Human insulin is being commercially produced from a transgenic species of:-
(1) Mycobacterium
(2) Rhizobium
(3) Saccharomyces
(4) Escherichia
Answer: 4
28. the main objective of production/use of herbicide-resistant GM crops is to:-
(1) Eliminate weeds from the field without the use of herbicides
(2) Encourage eco-friendly herbicides
(3) Reduce herbicide accumulation in food articles for health safety
(4) Eliminate weeds from the field without the use of manual labor
Answer: 4
29. Which one of the following is commonly used in the transfer of foreign DNA into crop plants?
(1) Penicillium expansum
(2) Trichoderma harzianum
(3) Meloidogyme incognita
(4) Agrobacterium tumefaciens
Answer: 4
30. Polyethylene glycol method is used for:-
(1) Energy production from sewage
(2) Gene transfer without a vector
(3) Biodiesel production
(4) Seedless fruit production
Answer: 2
31. Transgenic plants are the ones:-
(1) Grown in the artificial medium after hybridization in the field
(2) Produced by a somatic embryo in an artificial medium
(3) Generated by introducing foreign DNA into a cell and regenerating a plant from that cell
(4) Produced after protoplast fusion in an artificial medium
Answer: 3
32. The genetic defect - adenosine deaminase (ADA) deficiency may be cured permanently by:-
(1) Enzyme replacement therapy
(2) Periodic infusion of genetically engineered lymphocytes having functional ADA cDNA
(3) Administering adenosine deaminase activators
(4) Introducing bone marrow cells producing ADA into cells at early embryonic stages
Answer: 4
33. Which one of the following is used as a vector for cloning genes into higher organisms?
(1) Rhizopus nigricans
(2) Retrovirus
(3) Baculovirus
(4) Salmonella typhimurium
Answer: 2
34. Restriction endonucleases are enzymes which :
(1) restrict the action of the enzyme DNA polymerase
(2) remove nucleotides from the ends of the DNA molecule
(3) make cuts at specific positions within the DNA molecule
(4) recognize a specific nucleotide sequence for binding of DNA ligase
Answer: 3
35. The bacterium Bacillus thuringiensis is widely used in contemporary biology as:-
(1) Source of industrial enzyme
(2) Indicator of water pollution
(3) Insecticide
(4) Agent for production of dairy products
Answer: 3
36. What is true about Bt toxin?
(1) The concerned Bacillus has antitoxins
(2) The inactive protoxin gets converted into an active form in the insect gut
(3) Bt protein exists as an active toxin in the Bacillus
(4) The activated toxin enters the ovaries of the pest to sterilize it and thus prevent its multiplication
Answer: 2
37. If a recombinant DNA bearing the gene for ampicillin resistance is transferred into E.Coli cells and the host cells are spread on agar plates containing ampicillin, then________
(1) both transformed and untransformed recipient cells will die
(2) both transformed and untransformed recipient cells will be growing
(3) transformed recipient cells will grow and
untransformed recipient cells will die
(4) transformed recipient cells will die and
untransformed recipient cells will grow
Answer: 3
38. If hemoglobin (Hb) of a normal individual and a sickle-cell patient is run in an electrophoretic field, they will show:-
(1) same mobilities
(2) different mobilities
(3) Hb of the patient will not move at all
(4) Hbs are immobile
Answer: 2
39. In r-DNA technology or genetic engineering elution means_______
(1) Remove the DNA from the centrifuge tube after centrifugation
(2) The separated band of DNA are cut out from the gel and extracted from the gel piece
(3) Separation of the recombinant protein from recombinant cell
(4) Insertion of recombinant DNA into the host cell
Answer: 2
40. An example of gene therapy is_______
(1) Production of injectable Hepatitis–B vaccine
(2) Production of vaccines in food crops like potatoes which can be eaten
(3) Introduction of the gene for adenosine deaminase in persons suffering from severe combined immunodeficiency (SCID)
(4) Production of test-tube babies by artificial insemination and implantation of fertilized eggs
Answer: 3
41. Introduction of food plants developed by genetic engineering is not desirable because__________
(1) Economy of developing countries may suffer.
(2) These products are less tasty as compared to the already existing products.
(3) This method is costly.
(4) There is the danger of coming viruses and toxins with the introduced crop.
Answer: 4
42. Which one of the following is a correct statement
(1) "Bt" in "Bt-cotton" indicates that it is a genetically modified organism produced through biotechnology
(2) Somatic hybridization involves the fusion of two complete plant cells carrying desired genes.
(3) The anticoagulant hirudin is being produced from transgenic Brassica napus seeds.
(4) "Flavr Savr" variety of tomato has enhanced the production of ethylene which improves its taste.
Answer: 3
43. Which of the following tools of recombinant DNA technology is incorrectly paired with its use_______
(1) restriction enzyme - Production of RFLPs
(2) DNA ligase-that cuts DNA, creating the sticky ends
(3) DNA polymerase - used in a polymerase chain reaction to amplify section of DNA
(4) reverse transcriptase - production of cDNA from mRNA
Answer: 2
44. Select the incorrect statement for continuous culture system–
(1) In this used medium is drained out from one side while the fresh medium is added from another side.
(2) In this cells are maintained in their physiologically most active lag phase of growth.
(3) It produces larger biomass.
(4) It shows higher yields of the desired product.
Answer: 2
45. Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells):-
(1) an antifeedant
(2) a toxic protein
(3) both sense and anti-sense RNA
(4) a particular hormone
Answer: 3
46. The first clinical gene therapy was given for treating :
(1) Rheumatoid arthritis
(2) Adenosine deaminase deficiency
(3) Diabetes mellitus
(4) Chickenpox
Answer: 2
47. The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of :
(1) Inactivation of glycosidase enzyme in recombinant bacteria
(2) Non-recombinant bacteria containing beta-galactosidase
(3) Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
(4) Insertional inactivation of beta-galactosidase in recombinant bacteria
Answer: 4
48. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :
(1) Restriction mapping
(2) Centrifugation
(3) Polymerase chain reaction
(4) Electrophoresis
Answer: 4
49. Which one is a true statement regarding DNA polymerase used in PCR?
(1) It is isolated from a virus
(2) It remains active at high temperature
(3) It is used to ligate introduced DNA in recipient cells
(4) It serves as a selectable marker
Answer: 2
50. For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:-
(1) Silicon or Platinum
(2) Gold or Tungsten
(3) Silver or platinum
(4) Platinum or zinc
Answer: 2