Polymerase Chain Reaction MCQ with Answers:
1. In the process of using PCR technology to amplify the target gene, the undesired conditions are_________
A. primer
B. template DNA
C. ribonucleotides
D. thermostable DNA polymerase
Answer: C
2. Taq DNA polymerase is characterized by________
A. High temperature resistance
B. Strong acid resistance
C. Strong alkali resistance
D. heat labile
Answer: A
3. Compared with general DNA polymerase, Taq DNA polymerase has the following characteristics:
A. Efficient
B. specific
C. High temperature resistance
D. The catalytic sub-chain extends in the direction of 5′→3′
Answer: C
4. The chemical bond formed by DNA polymerase is______
A. hydrogen bonding
B. Phosphodiester bond
C. Peptide bond
D. high energy phosphate bond
Answer: B
5. The standard PCR technology process is generally divided into three steps: denaturation, renaturation, and extension. The temperatures required for these three steps are in order.
A. 92°C, 50°C, 72°C
B. 72°C, 50°C, 92°C
C. 50°C, 92°C, 72°C
D. 80°C, 50°C, 72°C
Answer: A
6. The most prominent advantage of PCR technology is that_________
A. The principle is simple
b. Easy to find raw materials
C. Taq DNA polymerase is thermostable
D. Fast, efficient, flexible and easy to operate
Answer: D
7. In PCR amplification of DNA fragments, the direction of DNA synthesis is_______
A. Extend from the 3' end of the daughter strand to the 5' end
B. Extend from the 5' end of the primer
C. Extend from the 5' end of the daughter strand to the 3' end
D. Any of the above
Answer: C
8. The role of primers is______
A. Open the DNA double strand
B. Catalyzes the synthesis of DNA daughter strands
C. Enables DNA polymerase to replicate from the 3' end of the primer
D. provide templates
Answer: C
9. Using PCR technology to amplify DNA, the required conditions are_________
① helicase
② primers
③ four kinds of deoxynucleotides
④ thermostable DNA polymerase
⑤ target gene
⑥ RNA polymerase
A. ②③④⑤
B. ①②③④
C．①③④⑤
D. ①②③⑥
Answer: A
10. Which of the following should be added to a microcentrifuge tube prior to PCR amplification?
① Template DNA
② Template RNA
③ DNA helicase
④ Heat-resistant DNA polymerase
⑤ Primer
⑥ PCR buffer
⑦ Deoxynucleotide stock solution
⑧ Nucleotide stock solution
A. ①④⑤⑥⑦
B. ①③④⑤⑥⑧
C．②③④⑤⑥⑦
D. ①④⑥⑦⑧
Answer: A
11. Which of the following statements about the PCR process and intracellular DNA replication is incorrect_______
A. The PCR process requires artificially synthesized short DNA or RNA single strands
B. The PCR process does not require a helicase, but does require a DNA polymerase
C. During PCR, the DNA can be replicated directly using the DNA double-strand as a template without unwinding
D. When DNA replicates in cells, DNA replicates while unwinding
Answer: C
12. Which of the following statements about PCR technology is incorrect?
A. PCR is a nucleic acid synthesis technique that replicates specific DNA fragments in vitro
B. It can be seen from the reaction process of PCR that phosphodiester bonds are more stable to high temperature than hydrogen bonds
C. The premise of using PCR technology to obtain the target gene is to have a known nucleotide sequence of the target gene
D. PCR amplification consumes four ribonucleotides and two primers
Answer: D
13. PCR is a technique for rapidly amplifying DNA fragments in vitro. The following statements about the PCR process are incorrect______
A. During the denaturation process, the hydrogen bonds between the base pairs in the DNA molecule are destroyed
B. In the process of renaturation, the binding of primers and DNA template strands is completed by the principle of base complementary pairing
C. The template and primers must be continuously added during the PCR reaction
D. The PCR reaction includes three cycles of denaturation, renaturation and extension
Answer: C
14. Primers are very important in PCR amplification experiments. The following are the characteristics of primers:
① The length of the primer is generally 80-100 base pairs (bp);
② The (G+C)/(A+T) content of the primer is generally 40%-60% ③ The 3' end of the primer There cannot be more than 3 consecutive identical bases ④ The primer itself cannot contain complementary sequences
A. ①②
B. ③④
C. ①③
D. All of these
Answer: B
15. Which of the following statements about PCR reactions is correct_______
A. The primers required for the PCR reaction are only RNA
B. The material required for the PCR reaction is ribonucleotides
C. Enzymes required for PCR reactions are denatured at 60°C
D. PCR reactions need to be carried out in a certain buffer solution
Answer: D
16. PCR technology is a technology that replicates specific DNA fragments in vitro. The operation steps of this technology are as follows:
A. High temperature denaturation, intermediate temperature extension, low temperature renaturation
B. High temperature denaturation, low temperature renaturation, medium temperature extension
C. Intermediate temperature extension, high temperature denaturation, low temperature renaturation
D. None of these
Answer: B